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Addgene inc gst blm
Gst Blm, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gst+blm/BKK24540+(Bacterial+strain+%2394799)/pmc04018780-197-7-40
Average 90 stars, based on 1 article reviews
gst blm - by Bioz Stars, 2026-09
90/100 stars

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Plasmid Preparation:

Article Title: Chk2 prevents mitotic exit when the majority of kinetochores are unattached
Article Snippet: The Mps1-GFP plasmid (see previous paragraph) was used to generate point mutations A862G, C863A, A864G (numbers refer to human Mps1 cDNA) changing Thr288 to glutamic acid (T288E), or mutation A862G changing Thr288 to alanine (T288A) by site-directed mutagenesis using the mutagenesis kit (QuikChange; Agilent Technologies). .. To generate plasmid pGEX BLM (9–479) coding GST-BLM (9–479) protein, a BLM fragment containing amino acids 9–479 of human BLM was isolated by PCR using pGEX-4T-1 BLM (1–1,417) as a template, cloned as a BamHI–NotI fragment into the pGEX-4T-1 vector (Addgene), and completely sequenced. .. To produce cell lines expressing WT or nonphosphorylatable S331A mutant Aurora B, WT or T1052G (changing S331 to alanine) human Aurora B cDNAs were subcloned as EcoRI–NotI fragments into the 6×Myc-pcDNA3 vector (Invitrogen), excised with BamHI–NotI, and introduced into the pcDNA5/FRT/TO plasmid (Invitrogen) as previously described ( ).

Isolation:

Article Title: Chk2 prevents mitotic exit when the majority of kinetochores are unattached
Article Snippet: The Mps1-GFP plasmid (see previous paragraph) was used to generate point mutations A862G, C863A, A864G (numbers refer to human Mps1 cDNA) changing Thr288 to glutamic acid (T288E), or mutation A862G changing Thr288 to alanine (T288A) by site-directed mutagenesis using the mutagenesis kit (QuikChange; Agilent Technologies). .. To generate plasmid pGEX BLM (9–479) coding GST-BLM (9–479) protein, a BLM fragment containing amino acids 9–479 of human BLM was isolated by PCR using pGEX-4T-1 BLM (1–1,417) as a template, cloned as a BamHI–NotI fragment into the pGEX-4T-1 vector (Addgene), and completely sequenced. .. To produce cell lines expressing WT or nonphosphorylatable S331A mutant Aurora B, WT or T1052G (changing S331 to alanine) human Aurora B cDNAs were subcloned as EcoRI–NotI fragments into the 6×Myc-pcDNA3 vector (Invitrogen), excised with BamHI–NotI, and introduced into the pcDNA5/FRT/TO plasmid (Invitrogen) as previously described ( ).

Polymerase Chain Reaction:

Article Title: Chk2 prevents mitotic exit when the majority of kinetochores are unattached
Article Snippet: The Mps1-GFP plasmid (see previous paragraph) was used to generate point mutations A862G, C863A, A864G (numbers refer to human Mps1 cDNA) changing Thr288 to glutamic acid (T288E), or mutation A862G changing Thr288 to alanine (T288A) by site-directed mutagenesis using the mutagenesis kit (QuikChange; Agilent Technologies). .. To generate plasmid pGEX BLM (9–479) coding GST-BLM (9–479) protein, a BLM fragment containing amino acids 9–479 of human BLM was isolated by PCR using pGEX-4T-1 BLM (1–1,417) as a template, cloned as a BamHI–NotI fragment into the pGEX-4T-1 vector (Addgene), and completely sequenced. .. To produce cell lines expressing WT or nonphosphorylatable S331A mutant Aurora B, WT or T1052G (changing S331 to alanine) human Aurora B cDNAs were subcloned as EcoRI–NotI fragments into the 6×Myc-pcDNA3 vector (Invitrogen), excised with BamHI–NotI, and introduced into the pcDNA5/FRT/TO plasmid (Invitrogen) as previously described ( ).

Clone Assay:

Article Title: Chk2 prevents mitotic exit when the majority of kinetochores are unattached
Article Snippet: The Mps1-GFP plasmid (see previous paragraph) was used to generate point mutations A862G, C863A, A864G (numbers refer to human Mps1 cDNA) changing Thr288 to glutamic acid (T288E), or mutation A862G changing Thr288 to alanine (T288A) by site-directed mutagenesis using the mutagenesis kit (QuikChange; Agilent Technologies). .. To generate plasmid pGEX BLM (9–479) coding GST-BLM (9–479) protein, a BLM fragment containing amino acids 9–479 of human BLM was isolated by PCR using pGEX-4T-1 BLM (1–1,417) as a template, cloned as a BamHI–NotI fragment into the pGEX-4T-1 vector (Addgene), and completely sequenced. .. To produce cell lines expressing WT or nonphosphorylatable S331A mutant Aurora B, WT or T1052G (changing S331 to alanine) human Aurora B cDNAs were subcloned as EcoRI–NotI fragments into the 6×Myc-pcDNA3 vector (Invitrogen), excised with BamHI–NotI, and introduced into the pcDNA5/FRT/TO plasmid (Invitrogen) as previously described ( ).



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